| Start: Depart University of Louisville (college), Louisville, Kentucky on Local road(s) (East) | 0.1 |
| 1: Bear LEFT (East) onto Intramural Pl | < 0.1 |
| 2: Road name changes to E Warnock St | 0.2 |
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| 3: Take Ramp (RIGHT) onto I-65 towards I-65 / Nashville | 166.8 |
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| 5: Construction near Goodlettsville (SB) (December 31, 2007 - July 17, 2010) | |
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| 6: Merge onto I-24 [I-65] | 4.5 |
| 7: At exit 50B, take Ramp (RIGHT) onto I-40 towards I-40 / I-65 S / Memphis / Huntsville | 1.0 |
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| 8: At exit 210 B, take Ramp (LEFT) onto I-65 towards I-65 / Huntsville | 188.7 |
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| 9: Reconstruction near Elkton (SB) (May 4, 2009 - September 15, 2010) | |
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| 11: Construction near Indian Trace (SB) (January 30, 2009 - November 9, 2009) | |
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| 12: Reconstruction near Birmingham (SB) (December 10, 2007 - November 30, 2010) | |
| 13: At exit 261B, take Ramp (RIGHT) onto I-20 [I-59] towards I-20 / I-59 / Tuscaloosa | 148.3 |
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| 14: Resurfacing near Tuscaloosa (WB) (October 5, 2007 - November 13, 2009) | |
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| 16: Keep LEFT onto I-59 towards I-59 / Laurel / New Orleans | 83.2 |
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| 17: Construction near Laurel (WB) (October 6, 2008 - November 30, 2009) | |
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| 18: Resurfacing in Moselle (SB) (July 1, 2009 - November 30, 2009) | |
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| 19: At exit 65, turn RIGHT onto Ramp towards US-98 / Hardy St / Columbia | 0.2 |
| 20: Keep LEFT to stay on Ramp towards Hardy St. / University of Southern Miss | < 0.1 |
| 21: Bear LEFT (South) onto Local road(s) | < 0.1 |
| 22: Turn LEFT (East) onto SR-198 [Hardy St] | 1.1 |
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| 23: Keep STRAIGHT onto SR-198 [W Hardy St] | 0.1 |
| 24: Turn LEFT (North) onto W Memorial Dr | 0.2 |
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| End: Arrive College Dr, Hattiesburg, MS 39401 | |
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