| Start: Depart University of Alabama at Birmingham (college), Birmingham, Alabama on SR-149 [University Blvd] (West) | 0.3 |
| 1: Take Ramp (RIGHT) onto I-65 towards I-65 / Huntsville | 1.7 |
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| 2: Reconstruction near Birmingham (NB) (December 10, 2007 - November 30, 2010) | |
| 3: At exit 261B, take Ramp (LEFT) onto I-20 [I-59] towards I-20 / I-59 / Tuscaloosa | 148.2 |
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| 4: Resurfacing near Tuscaloosa (WB) (October 5, 2007 - November 13, 2009) | |
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| 6: Keep LEFT onto I-59 towards I-59 / Laurel / New Orleans | 83.2 |
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| 7: Construction near Laurel (WB) (October 6, 2008 - November 30, 2009) | |
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| 8: Resurfacing in Moselle (SB) (July 1, 2009 - November 30, 2009) | |
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| 9: At exit 65, turn RIGHT onto Ramp towards US-98 / Hardy St / Columbia | 0.2 |
| 10: Keep LEFT to stay on Ramp towards Hardy St. / University of Southern Miss | < 0.1 |
| 11: Bear LEFT (South) onto Local road(s) | < 0.1 |
| 12: Turn LEFT (East) onto SR-198 [Hardy St] | 1.1 |
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| 13: Keep STRAIGHT onto SR-198 [W Hardy St] | 0.1 |
| 14: Turn LEFT (North) onto W Memorial Dr | 0.2 |
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| End: Arrive College Dr, Hattiesburg, MS 39401 | |
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