| Start: Depart Knoxville, Tennessee, United States on US-441 [SR-33] (North) | 0.2 |
| 1: Keep RIGHT onto Ramp towards I-40 / Asheville | 0.3 |
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| 2: Keep LEFT to stay on Ramp towards I-275 / I-40 / Lexington / Nashville | 0.3 |
| 3: Take Ramp (RIGHT) onto I-40 towards I-40 / Nashville | 19.7 |
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| 4: Bridge work near Knoxville (WB) (May 9, 2008 - November 30, 2009) | |
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| 5: Keep LEFT onto I-75 towards I-75 / Chattanooga | 83.4 |
| 6: At exit 2, take Ramp (RIGHT) onto I-24 towards I-24 / I-59 / Chattanooga / Birmingham | 17.3 |
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| 8: At exit 167, take Ramp (LEFT) onto I-59 towards I-59 / Birmingham | 124.1 |
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| 10: At exit 137, take Ramp (LEFT) onto I-459 towards I-459 / Tuscaloosa / Montgomery | 33.2 |
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| 11: Interchange Reconstruction near Hoover (WB) (October 9, 2009 - May 28, 2010) | |
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| 12: Take Ramp (LEFT) onto I-20 [I-59] towards I-20 / I-59 / Tuscaloosa | 130.0 |
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| 13: Resurfacing near Tuscaloosa (WB) (October 5, 2007 - November 13, 2009) | |
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| 15: Keep LEFT onto I-59 towards I-59 / Laurel / New Orleans | 83.2 |
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| 16: Construction near Laurel (WB) (October 6, 2008 - November 30, 2009) | |
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| 17: Resurfacing in Moselle (SB) (July 1, 2009 - November 30, 2009) | |
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| 18: At exit 65, turn RIGHT onto Ramp towards US-98 / Hardy St / Columbia | 0.2 |
| 19: Keep LEFT to stay on Ramp towards Hardy St. / University of Southern Miss | < 0.1 |
| 20: Bear LEFT (South) onto Local road(s) | < 0.1 |
| 21: Turn LEFT (East) onto SR-198 [Hardy St] | 1.1 |
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| 22: Keep STRAIGHT onto SR-198 [W Hardy St] | 0.1 |
| 23: Turn LEFT (North) onto W Memorial Dr | 0.2 |
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| End: Arrive College Dr, Hattiesburg, MS 39401 | |
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