| Start: Depart 2624 Clifton Ave, Cincinnati, OH 45220 on Clifton Ave (North) | 0.3 |
| 1: Turn LEFT (West) onto Martin Luther King Dr (W) | 0.9 |
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| 2: Road name changes to US-52 [Hopple St] | 0.1 |
| 3: Take Ramp (RIGHT) onto I-75 towards I-75 | 3.3 |
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| 4: Merge onto I-71 [I-75] | 19.2 |
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| 6: At exit 173, take Ramp (RIGHT) onto I-71 towards I-71 / Louisville | 71.6 |
| 7: At exit 5, take Ramp (LEFT) onto I-264 towards I-264 / Watterson Expressway | 10.4 |
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| 8: At exit 12, turn RIGHT onto Ramp towards I-65 / KY-61 / Preston Highway / Nashville / Indianapolis | 0.1 |
| 9: Keep LEFT to stay on Ramp towards I-65 / Nashville / Downtown Louisville / Indianapolis | 0.2 |
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| 10: Take Ramp (LEFT) onto I-65 towards I-65 / Nashville | 165.1 |
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| 12: Construction near Goodlettsville (SB) (December 31, 2007 - July 17, 2010) | |
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| 13: Merge onto I-24 [I-65] | 4.5 |
| 14: At exit 50B, take Ramp (RIGHT) onto I-40 towards I-40 / I-65 S / Memphis / Huntsville | 1.0 |
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| 15: At exit 210 B, take Ramp (LEFT) onto I-65 towards I-65 / Huntsville | 188.7 |
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| 16: Reconstruction near Elkton (SB) (May 4, 2009 - September 15, 2010) | |
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| 18: Construction near Indian Trace (SB) (January 30, 2009 - November 9, 2009) | |
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| 19: Reconstruction near Birmingham (SB) (December 10, 2007 - November 30, 2010) | |
| 20: At exit 261B, take Ramp (RIGHT) onto I-20 [I-59] towards I-20 / I-59 / Tuscaloosa | 148.3 |
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| 21: Resurfacing near Tuscaloosa (WB) (October 5, 2007 - November 13, 2009) | |
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| 23: Keep LEFT onto I-59 towards I-59 / Laurel / New Orleans | 83.2 |
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| 24: Construction near Laurel (WB) (October 6, 2008 - November 30, 2009) | |
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| 25: Resurfacing in Moselle (SB) (July 1, 2009 - November 30, 2009) | |
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| 26: At exit 65, turn RIGHT onto Ramp towards US-98 / Hardy St / Columbia | 0.2 |
| 27: Keep LEFT to stay on Ramp towards Hardy St. / University of Southern Miss | < 0.1 |
| 28: Bear LEFT (South) onto Local road(s) | < 0.1 |
| 29: Turn LEFT (East) onto SR-198 [Hardy St] | 1.1 |
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| 30: Keep STRAIGHT onto SR-198 [W Hardy St] | 0.1 |
| 31: Turn LEFT (North) onto W Memorial Dr | 0.2 |
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| End: Arrive College Dr, Hattiesburg, MS 39401 | |
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