| Start: Depart University of South Florida (college), Tampa, Florida on Local road(s) (South) | 0.1 |
| 1: Turn LEFT (East) onto Local road(s) | < 0.1 |
| 2: Turn RIGHT (South) onto Leroy Collins Blvd | 0.4 |
| |
 |
| 3: Turn RIGHT (West) onto SR-582 [E Fowler Ave] | 2.5 |
| |
|
| 4: Take Ramp (RIGHT) onto I-275 towards I-275 / Ocala | 8.6 |
| 5: Take Ramp onto I-75 | 162.1 |
| |
|
| 6: At exit 435, turn off onto Ramp towards I-10 / Jacksonville / Tallahassee | 0.2 |
| 7: Take Ramp (LEFT) onto I-10 towards I-10 / Tallahassee | 334.6 |
| |
|
| 8: Construction near Tallahassee (WB) (December 1, 2006 - November 30, 2009) | |
| |
|
| |
|
| 10: At exit 27, turn RIGHT onto Ramp towards US-90 / US-98 / Government St | 0.4 |
| |
|
| 11: Keep LEFT to stay on Ramp | < 0.1 |
| 12: Keep STRAIGHT onto Local road(s) | < 0.1 |
| 13: Road name changes to US-98 [SR-16] | 1.5 |
| |
|
| 14: Turn RIGHT (North) onto US-90 [US-98] | 0.3 |
| 15: Turn LEFT (West) onto US-98 [St Anthony St] | 3.6 |
| |
|
| 16: Keep RIGHT onto Local road(s) | 0.1 |
| 17: Keep STRAIGHT onto US-98 [SR-42] | 83.8 |
| |
|
| |
|
| 19: Keep RIGHT onto Ramp towards US-49 / Hattiesburg / Gulfport | 0.2 |
| 20: Take Ramp (RIGHT) onto US-49 towards Hattiesburg | 6.4 |
| |
|
| 21: Bridge work near Hattiesburg (NB) (July 10, 2009 - April 9, 2010) | |
| |
|
| 22: Turn LEFT (West) onto SR-198 [Hardy St] | < 0.1 |
| 23: Keep STRAIGHT onto SR-198 [W Hardy St] | 0.2 |
| 24: Turn RIGHT (North) onto W Memorial Dr | 0.2 |
| |
|
| End: Arrive College Dr, Hattiesburg, MS 39401 | |
| |
|
